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Rapid test for the preclinical postmortem diagnosis of BSE in central nervous system tissue
  1. J. Grassi, PhD1,
  2. S. Simon, PhD1,
  3. C. Crminon, PhD1,
  4. Y. Frobert, PharmD, PhD, CEA1,
  5. E. Comoy, DVetMed, PhD2,
  6. S. Trapmann, PhD3,
  7. H. Schimmel, PhD3,
  8. S. A. C. Hawkins, MIBiol4,
  9. G.A. H. Wells, BVetMed, FRCPath, MRCVS4,
  10. J. Moynagh, MVB, DLS, DipStat, MRCVS5 and
  11. J. P. Deslys, MedD, PhD, CEA6
  1. 1 Pharmacology and Immunology Unit, CEA/Saclay, 91191 Gif sur Yvette cedex, France
  2. 2 Bio-Rad Life Science Laboratories, Hercules, California 94547, USA and Marnes La Coquette, France
  3. 3 European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg, B2440 Geel, Belgium
  4. 4 Veterinary Laboratories Agency Weybrdge, New Haw, Addlestone, Surrey KT15 3NB
  5. 5 Directorate General Health and Consumer Protection, European Commission, Rue Belliard 232, 1049 Brussels, Belgium
  6. 6 Neurovirology Unit, CEA/Fontenay aux Roses, BP6 92265 Fontenay aux Roses, France

Abstract

The efficacy of a rapid test for detecting PrPSc in central nervous system tissue was evaluated for the postmortem diagnosis of BSE at different times during the course of the disease. One hundred and six samples of brain, at the level of the medulla oblongata, and spinal cord, derived from the experimental study of the pathogenesis of BSE carried out in Great Britain between 1991 and 1995, were examined. PrPSc was detected in the samples from most of the exposed animals killed 32 months or more after they had been exposed to the agent, and before the onset of clinical signs which were first recorded at 35 months. Comparisons with the results of histology, fibril detection, PrP immunohistochemistry and mouse bioassay indicated that the rapid test is at least as sensitive as these conventional confirmatory diagnostic methods and its result can be obtained more quickly.

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