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Presence of Mycobacterium avium subspecies paratuberculosis in suspensions of ovine trichostrongylid larvae produced in faecal cultures artificially contaminated with the bacterium
  1. J. B. Lloyd, DVM, MVSt, PhD1,
  2. R. J. Whittington, BVSc, PhD, MACVSc1,
  3. C. Fitzgibbon, BAppSc1 and
  4. R. Dobson, BAppSc, PhD2
  1. 1 NSW Agriculture, Elizabeth Macarthur Agricultural Institute, Private Mailbag 8, Camden NSW, Australia 2570
  2. 2 CSIRO McMaster Laboratory, Locked Bag 1, Blacktown NSW, Australia 2148
  1. Dr Lloyd's present address is Pfizer Animal Health, PO Box 57, West Ryde NSW, Australia 2114

Abstract

A reference strain of Mycobacterium avium subspecies paratuberculosis was added to faecal larval cultures of Haemonchus contortus, Ostertagia circumcincta and Trichostrongylus colubriformis. Samples of the larvae produced were cultured for the presence of the bacterium in modified BACTEC 12B medium, both before and after exposure to gamma irradiation. The water used to wash the larvae off the faecal cultures was also tested for the presence of the bacterium. Positive growth was confirmed as M avium subspecies paratuberculosis by IS900 polymerase chain reaction and restriction endonuclease analysis of the product. M avium subspecies paratuberculosis was detected in the unirradiated larval suspensions and wash waters of all three nematode species, and in the irradiated H contortus larval suspension.

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