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In vitro mucolytic activity of recombinant human deoxyribonuclease on equine tracheobronchial mucus
  1. M. Pietra, DVM1,
  2. C. Guglielmini, DVM1,1,
  3. S. Cinotti, DVM1 and
  4. M. Forni, DBS, PhD2
  1. 1 Department of Veterinary Clinical Sciences
  2. 2 Department of Veterinary Morphophysiology and Animal Production, School of Veterinary Medicine, University of Bologna, Via Tolara di Sopra 50,40064 Ozzano Emilia (BO), Italy

Abstract

The viscosity of the mucus, its DNA concentration and the size range of the DNA were determined on tracheobronchial samples from 11 horses with lower airway diseases before and after incubation with recombinant human deoxyribonuclease (rhDNase). The horses were divided into two groups on the basis of the cytology of the samples: group A (five horses) with more than 60 per cent neutrophils and group B (six horses) with fewer than 50 per cent neutrophils. The mean mucus viscosity and DNA concentration in the preincubation samples were significantly higher in group A than in group B, and there was a correlation between DNA concentration and mucus viscosity in the preincubation samples from group A. Incubation with rhDNase significantly reduced the viscosity of the samples only in group A.

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