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Serodiagnosis of goat warble fly infestation by Przhevalskiana silenus with a commercial ELISA kit
  1. D. Otranto, DVM1,
  2. C. Boulard, DB2,
  3. A. Giangaspero, DVM1,
  4. M. P. Caringella, DB1,
  5. D. Rimmele, PhD3 and
  6. V. Puccini, DVM1
  1. 1 Dipartmento di Sanità Benessere Animale, Facoltá di Medicina Veterinaria, Università Degli studi di Bari, Valenzano, 70010, Italy
  2. 2 Laboratoire d' Immunopathologie des Maladies Parasitaires, INRA, Nouzilly, 37380, France
  3. 3 Diagnostic Department, Wtoquinol Group, Lure Cedex, 70204, France


Cross-reactivity between Hypoderma lineatum antigen and anti-Przhevalskiana silenus antibodies has been demonstrated by an ELISA technique. To evaluate the applicability of a commercial ELISA kit for the immunodiagnosis of goat warble fly infestation, different dilutions of serum and conjugate were tested, the development of antibody to P silenus in naturally infested goats was studied, and the results were compared with an ELISA technique using an antigen extracted from the first instar larvae of H lineatum. The best results were obtained with a serum dilution of 1:50; with both techniques the highest antibody concentration were recorded in October, November and December. In view of the confirmed cross-reactivity between H lineatum antigen and anti-P silenus antibodies, and the simplicity and rapidity of the assay, the commercial ELISA kit can be considered as a useful tool for the diagnosis of goat warble fly infestation.

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