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Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis in sheep
  1. C. M. Marín, DVM, PhD1,
  2. B. Alonso-Urmeneta, PhD2,
  3. I. Moriyón, PhD2,
  4. S. Pérez-Gómez, DVM3 and
  5. J. M. Blasco, DVM, PhD1
  1. 1 Unidad de Sanidad Animal, Servicio de Investigación, Agroalimentaria, Diputación General de Aragón, Ap 727, 50080 Zaragoza, Spain
  2. 2 Departamento Microbiología, Universidad de Navarra, Ap 177, 30080, Pamplona, Spain
  3. 3 Laboratorio Pecuario, Gobierno de Navarra, Serapio Huici s.n., 31610, Villaba, Spain

Abstract

The sensitivities of three commercial peroxidase conjugates (polyclonal anti-sheep IgG, recombinant protein G and a monoclonal anti-ruminant IgG1) in an enzyme-linked immunosorbent assay for Brucella ovis were evaluated. The monoclonal and protein G conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella-free sheep. The protein G conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion test. Both tests were highly specific when testing sera from brucella-free animals but detected as positive a large proportion of sera from both Brucella melitensis-infected and B melitensis Rev 1-vaccinated sheep.

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