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Detection of Newcastle disease virus-specific antibodies in ostrich sera by three serological methods
  1. G. Koch, DVM, PhD1,
  2. G. Czifra, DVM2 and
  3. B. E. Engström, DVM, PhD, SVA2
  1. 1 ID-DLO, Institute of Animal Science and Health, Departnent of Avian Virology, PO Box 365, 8200 Ai Lelystad, The Netherlands
  2. 2 The National Veterinary Institute, Departnent of Poultry, PO Box 7073, S-75007 Uppsala, Sweden
  1. The National Veterinary Institute, Departnent of Poultry, PO Box 7073, S-75007 Uppsala, Sweden

Abstract

Sera from 211 ostriches were tested for the presence of Newcastle disease virus (NDv)-specific antibodies by the virus neutralisation test, the haemagglutination inhibition (HI) test and a recently developed avian paramyxovirus serotype 1 (PMv-1) specific monoclonal antibody blocking ELISA (b-ELISA). The virus neutralisation test was used as the reference for the estimation of the sensitivity and specificity of the b-ELISA and HI tests. Of the 211 sera, 140 contained NDv-specific neutralising antibodies, 130 were positive by the HI test and 122 by the b-ELISA. The sensitivity, specificity and predictive accuracy of the HI and b-ELISA tests relative to the virus neutralisation test were similar. The good agreement between the HI and b-ELISA test (κ = 0.85) suggested that the two methods are interchangeable.

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