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Laparoscopic recovery of pronuclear-stage goat embryos
  1. B. Kühholzer1,
  2. S. Müller2,
  3. U. Besenfelder2,
  4. M. I. Prokofiev3,
  5. L. K. Ernst4 and
  6. G. Brem1
  1. 1 LBI of Immuno-, Cyto- and Moleculargenetic Research, Vienna, Austria
  2. 2 Institute of Animal Breeding and Genetics, University of Veterinary Science, Vienna, Austria
  3. 3 Biotechcentre, Gorki-Leninski, Russia
  4. 4 Russian Academy of Agricultural Science, Moscow, Russia

Abstract

The oviducts of 16 Saanen does, superovulated with folliclestimulating hormone (FSH) and synchronised with prostaglandin F2α were flushed 75 to 86 hours after the injection of prostaglandin. The mean (sd) ovulation rate was 13.7 (3.9). The flushings were directed orthograde through a flexible intravenous catheter, which was introduced into the oviduct via the infundibulum. The flushing medium was recovered by a balloon-catheter, which was placed in the uterine lumen near the uterotubal junction. Five does were flushed unilaterally either because they had one blocked oviduct or because they had ovulated on only one ovary. The overall embryo recovery rate was 72 per cent. Nine weeks later 11 of the donor ewes were examined laparoscopically and no adhesions of the reproductive organs were observed. Eight of these does were synchronised with progestagen-vaginal sponges, superovulated with FSH and their oviducts were flushed again. Their mean ovulation rate was 16.0 (4.3) and 86 per cent of the embryos were recovered. The optimal time to obtain pronuclear stage embryos was 75 to 78 hours after the injection of prostaglandin. All the embryos recovered within this period were at the pronuclear stage whereas 28 per cent of those recovered one to six hours later were at the two- or four-cell stage.

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