Article Text

PDF
Detection of peste des petits ruminants virus antigen in conjunctival smears of goats by indirect immunofluorescence
  1. K. J. Sumption, PhD, MA, VetMB, MRCVS1,
  2. G. Aradom, DVM, MSc2,
  3. G. Libeau, PhD, MSc, CIRAD-EMVT3 and
  4. A. J. Wilsmore, PhD, BVSc, DipAH, MRCVS2,1
  1. 1 Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG
  2. 2 Animal Resources Department, Ministry of Agriculture, PO Box 1162, Asmara, Eritrea
  3. 3 Campus de Baillarguet, Montpellier, France

Abstract

Peste des petits ruminants virus (PPRv) antigen was detected in conjunctival epithelial cells obtained from goats in the early or late stage of the disease by the use of a specific monoclonal antibody (mAb) to PPRV in an immunofluorescent antibody test (EFAT). The affected goats were sampled during an outbreak of peste des petits ruminants in Eritrea. Syncytia were also observed in some smears, consistent with a morbillivirus infection, but the IFAT was more sensitive than staining for syncytia in the detection of viral antigen, the two tests giving 63 per cent and 40 per cent of animals, respectively, with positive tests. Positive immunofluorescence was observed in samples from goats in the early and late stages of the disease, but was not observed with a specific rinderpest mAb or with conjunctival smears from uninfected animals. It is concluded that preparation of conjunctival smears for staining for syncytia is a simple procedure which can be applied in the field, and by use of a specific mAb PPRV infection can be rapidly confirmed and differentiated from rinderpest.

    Statistics from Altmetric.com

      Footnotes

      • Dr Wilsmore's present address is VEERU, University of Reading, Department of Agriculture, Earley Gate, PO Box 236, Reading RG6 6AT

      Request permissions

      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.