Activation of extracellular matrix metalloproteinases in equine laminitis
- P. J. Johnson, BVSc, MS, MRCVS1,
- S. C. Tyagi, PhD4,1,
- L. C. Katwa, PhD4,
- V. K. Ganjam, BVSc, PhD2,
- L. A. Moore, DVM, MS1,
- J. M. Kreeger, DVM, PhD3 and
- N. T. Messer, DVM1
Samples of connective tissue obtained from the hoof of six laminitic and eight non-laminitic adult horses were analysed zymographically to investigate whether connective tissue matrix metalloproteinases are activated or induced during laminitis. The activity of matrix metalloproteinases was substantially greater in the tissues from the laminitic horses than in the tissues from the non-laminitic horses. A comparison of the collagenolytic activity in the laminitic and control tissues showed that collagenolytic activities corresponding to the 92 kDa (P<0.001), 72 kDa (P<0.01) and 66 kDa (P<0.01) bands were induced in the laminitic tissues.
↵Dr Tyagi's present address is Department of Physiology and Biophysics, University of Mississippi Medical Center, 2500 North State Street, Jackson, Mississippi 39216-4505, USA
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