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Development of a slide ELISA for canine leishmaniasis and comparison with four serological tests
  1. F. Vercammen, DVM1,
  2. D. Berkvens, PhD1,
  3. D. Le Ray, DSc2,
  4. D. Jacquet2 and
  5. T. Vervoort, ClinChem3
  1. 1 Veterinary Department, Institute of Tropical Medicine, 2000 Antwerp, Belgium
  2. 2 Laboratory of Protozoology, Institute of Tropical Medicine, 2000 Antwerp, Belgium
  3. 3 Central Laboratory, Institute of Tropical Medicine, 2000 Antwerp, Belgium

Abstract

A slide ELISA for canine leishmaniasis was developed by using promastigotes of Leishmania infantum, and compared with microimmunodiffusion, immunoelectrophoresis, direct agglutination and indirect immunofluorescence assays. The sensitivity of all the tests was 100 per cent. The specificity of the direct agglutination test was 95 per cent but it was 100 per cent for the three other tests. There was also a positive correlation and a high level of concordance between the titres measured by the different tests.

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