Veterinary Record 141:303-306 doi:10.1136/vr.141.12.303
  • Papers and Articles

Molecular and clinicopathological diagnosis of malignant catarrhal fever in cattle, deer and buffalo in New Zealand

  1. K. M. Tham, DVM, PhD1
  1. 1 Virology Section, Central Animal Health Laboratory, MAF Quality Management, PO Box 40063, Upper Hutt, New Zealand


Fresh and formalin-fixed tissues and blood samples in ethylenediaminetetraacetate were collected from cattle, deer and buffalo with clinical signs suggestive of malignant catarrhal fever (MCF). In addition, formalin-fixed, paraffi'nembedded tissue blocks collected from these animals and retrospectively from field cases of MCF were examined. DNA samples extracted from these samples were analysed by polymerase chain reaction (PCR) assay using primers specific for the sheepassociated (SA)- and wildebeest-associated (WA)-MCF viruses. Both the SA-MCF virus and WA-MCF virus PCR yielded positive results which were in nearly complete agreement with the histopathological diagnoses of MCF in fresh and formalin-fixed, paraffin-embedded tissue from 29 cattle, 24 deer and three buffaloes. Some blood samples tested by the two assays indicated that some of the infected cattle were possible carriers.

    Sign up for job alerts

    Vet Record Careers features a comprehensive database of vet jobs.
    Browse for your next job, or set personalised job alerts so you hear about vacancies as soon as they go online.
    Find out more at Vet Record Careers

    Free Sample Issue

    This recent issue is free to all users to allow everyone the opportunity to see the full scope and typical content of the Veterinary Record.
    View free sample issue >>

    Navigate This Article