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High sensitivity polymerase chain reaction assay for active and latent feline herpesvirus- infections in domestic cats
  1. B. J. Weigler, DVM, MPH, PhD1,
  2. C. A. Babineau, MLT1,
  3. B. Sherry, PhD2 and
  4. M. P. Nasisse, DVM, MS1,1
  1. 1 Department of Companion Animal and Special Species Medicine
  2. 2 Department of Microbiology, Pathology and Parasitology, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina, USA, 27606

Abstract

A polymerase chain reaction (PCR) assay was developed and used to detect feline herpesvirus-1 (FHV-1) in conjunctival and oropharyngeal swabs, and in latently infected tissues (trigeminal ganglia, optic nerves, optic chiasma, olfactory bulbs and corneas) collected from 10 experimentally infected cats. There was good agreement between parallel tests of the swab specimens by PCR and virus isolation assay during the phase of acute, latent and recurrent disease episodes (kappa=0.63, P<0.001). The PCR reliably detected ≤240 copies of FHV-1 template DNA, significantly improving upon previously published PCR assays for the agent.

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      Footnotes

      • Dr Nasisse's present address is Department of Veterinary Medicine and Surgery, University of Missouri College of Veterinary Medicine, Columbia, Missouri, USA, 65211

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