Article Text

PDF
Comparison of Chlamydia psittaci from cats with upper respiratory tract disease by polymerase chain reaction analysis of the ompA gene
  1. J. E. Sykes, BVSc1,
  2. V. P. Studdert, BS, DVM2,
  3. G. Anderson, BAgrSci, GradDipAppIStats2 and
  4. G. F. Browning, BVSc, DipVetClinStud, PhD1
  1. 1 The University of Melbourne, Department of Veterinary Science, Veterinary Preclinical Centre, corner of Flemington Road and Park Drive, Parkville, Victoria 3052, Australia
  2. 2 The University of Melbourne, Veterinary Clinical Centre, 250 Princes Highway, Werribee, Victoria 3030, Australia

Abstract

Conjunctival swabs were taken from 168 cats with clinical signs of acute or chronic upper respiratory tract disease and tested for Chlamydia psittaci by the polymerase chain reaction (PCR) to amplify the ompA gene coding region. Twenty-two (13 per cent) were positive for C psittaci. The PCR products from positive samples were subjected to restriction endonuclease analysis with the restriction enzymes Alu I and Mse I. The fragments of DNA were detected on silver-stained polyacrylamide gels and the results were compared with the results obtained from chlamydial isolates from cats in Japan, France, the USA and the UK. All the strains had identical restriction patterns. When PCR is used as an epidemiological tool, feline chlamydial strains worldwide appear very similar

    Statistics from Altmetric.com

      Request permissions

      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.