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Partial identification of spirochaetes from two dairy cows with digital dermatitis by polymerase chain reaction analysis of the 16S ribosomal RNA gene
  1. S. G. T. Rijpkema, BSc, PhD1,
  2. G. P. David, BVSc, MRCVS2,
  3. S. L. Hughes, BSc, MSc1 and
  4. M. J. Woodward, BSc, PhD1
  1. 1 Molecular Genetics Unit, Department of Bacteriology, Central Veterinary Laboratory, Woodham Lane, Addlestone, Surrey KT15 3NB
  2. 2 Veterinary Information Centre, Kendal Road, Harlescott, Shrewsbury SY1 4HD
  1. Molecular Genetics Unit, Department of Bacteriology, Central Veterinary Laboratory, Woodham Lane, Addlestone, Surrey KT15 3NB

Abstract

Specimens taken postmortem from typical lesions of digital dermatitis in two dairy cows were tested by the polymerase chain reaction (PCR) for the presence of a spirochaetal 16S rRNA gene. Seven different assays detected the gene in the samples from both cows. Two of the PCR products were sequenced and a comparison of the nucleotide sequences revealed that the spirochaete belonged to the genus Treponema and was closely related to Treponema denticola. A PCR specific for the detection of the digital dermatitis-associated treponeme was developed.

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