Article Text

Development of a sperm zona pellucida binding assay for bull semen
  1. AR Fazeli,
  2. W Steenweg,
  3. MM Bevers,
  4. FA de Loos,
  5. J van den Broek and
  6. B Colenbrander

Abstract

Assays based on sperm zona pellucida binding have been developed as diagnostic tests to predict the fertilisation potential of human spermatozoa. The aim of this study was to establish a similar assay for bull sperm. The results showed differences between established fertile bulls in the relative numbers of sperm cells bound to the zona pellucida of a batch of oocytes. These differences suggest that there may be a relationship between the sperm zona pellucida binding capacity and the fertility of bulls.

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