Article Text

Cryopreservation of buffalo semen
  1. K Ahmad and
  2. RA Chaudhry

Abstract

Twenty-one split semen samples from five bulls were diluted in five diluents. The diluents comprised lactose-egg yolk-glycerol, fructose-egg yolk-glycerol and three different combinations of lactose and fructose with equal proportions of egg yolk and glycerol. The straw-method of deep freezing was used. Frozen semen was evaluated on the basis of post thawing motility percentage, survival of spermatozoa at 37 degrees C and absolute index of survival. Statistical analysis of data revealed highly significant differences between diluents for the three characteristics tested. The diluent comprising 11 per cent lactose 56.25 ml + 6 per cent fructose 18.75 ml + egg yolk 20 ml + glycerol 5 ml achieved the best results.

    Statistics from Altmetric.com

      Request permissions

      If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.